DATF Database of Transcription Factors
Datf stands for practicality. It stands for realism. It is an acronym for the five principles that shape the way we approach problems. DATF will collaborate with community partners to reduce youth use of marijuana, alcohol, or other drugs. These principles guide the work of DATF. In other words, Datf is a practical person who wants to build others up rather than tear them down.
The DATF database contains unique information about transcription factors found in Arabidopsis. DATF actually contains cloned sequences of 1177 genes. Of those sequences, 31 were different than previously reported. This information can also be searched by using DATF’s clone field. Annotations for a variety of Arabidopsis-specific transcription factor types are also available in DATF. It also contains information about the functional roles of each transcription factor.
The DATF data can be accessed online by family members and chromosomes. Clicking on the name of a family will provide an introduction to that family. You can also search by AGI locus ID, or via DATF’s advanced searching page. BLAST searches can be run against sequences in DATF’s database. The DATF website encourages users to submit new data for inclusion in the database. The DATF website also allows users to download raw data.
Studies with dATF-2 knockdown flies have shown that dPEPCK gene expression was decreased in these flies. PEPCK is an enzyme that plays a crucial role in the pathways of glucoseoneogenesis and gluconeogenesis. Therefore, PEPCK activity in the dATF-2 knockout fly would decrease the blood triglyceride and hemolymph levels.
In Drosophila, a homolog of ATF-2 (dATF-2) has been identified. This protein is directly phosphorylated by dp38b but not by dJNK. Genetic analyses have indicated that dATF-2 functions in the dp38 signaling pathway. An analysis of DNA arrays has also revealed that dATF-2 functions as a transcriptional activator for osmotic stress-inducible gene genes.
Dp38 phosphorylates dATF-2 at Thr-59 and Thr-61. This phosphorylation is inhibited by the p38-specific inhibitor SB203580. These results indicate that dATF is essential for the regulation of the p38-mediated stress response. It is critical for the normal functioning of the dp38 signaling pathway in the body. The normal development of brain cells and endocrine cell is affected by the presence of dATF-2.